The waterfall is a recurring location throughout the franchise. It was either a part of the challenge for the contestants, or a background location. The most notable waterfall is located at Camp Wawanakwa, appearing in four seasons.intro, Leshawna and Heather are seen slapping each other while sitting in a raft until they fall down the waterfall. Directly in front of the waterfall, Harold is seen practicing some sort of fighting, until Izzy swings on a vine and crashes into him.
In Who Can You Trust?, Gwen and Leshawna land on a waterfall after flying from a rock-slide.
In Jamaica Me Sweat, the first half of the challenge takes part on a waterfall. The teammates must tag each other, then one at a time dive into the lake and search for Chef's gold karaoke chains, while watching out for electric eels and sharks.
In Niagara Brawls, the beginning proceeds with the contestants falling on some swan boats out of the plane, dangerously having to escape out of the waterfall. The waterfall also takes a huge part in the second half of the challenge, where the couples must walk over Niagara Falls, on a tightrope, with the grooms carrying their brides, and for a dangerous twist, Chris added some sharks to the water below the Falls. The challenge starts, and Heather and Alejandro argue over who will be eliminated. After Heather's bickering, Alejandro "accidentally" loses his balance, sending him and Heather falling into the waterfall. After Sierra and Cody are rejected by the Chef, the two then run into Owen and Blaineley on the tightrope. As neither of them wish to move back, they all end up falling into the water after Sierra kicks Owen in the knee and pulls Blaineley's hair extensions for upsetting Cody. In the end, all the contestants except for Duncan and Courtney try to escape from the sharks, while Owen swims carrying them all.
In Hawaiian Style, when Bridgette explains the challenge, a waterfall can be seen in the background.
In Bigger! Badder! Brutal-er!, the waterfall appears where both of the teams have to jump over it.
In the exclusive clip of The Bold and the Booty-ful, Gwen and Scott ended up at Niagara Falls after taking the Flush of Shame. The clip ends with the two of them falling off the waterfall, seconds after realizing where they are.
Pahkitew Island also has a waterfall. It can be assumed that the waterfall is artificial, similar to the rest of the island.
In My Way or Zimbabwe, the teams must take a raft down the waterfall in Zimbabwe and take a selfie of them and their partners.
On one of promotional pictures, all of the main contestants are seen on a raft about to fall off the waterall.
During Total Drama World Tour and the spin-off series, a few waterfalls that exist in real life appear as well, those being Niagara Falls and Victoria Falls. Dunn's River Falls are also mentioned in Jamaica Me Sweat, though it is not visited.