The horse is an animal that makes several appearances throughout the series. The most recurring horse is a brown, short-legged horse with flies flying around it.
A horse makes an appearance as one of the animals shot by Izzy during Wawanakwa Gone Wild!. Its appearance is much different compared to the other horses in the series. This one is cream colored and has stars on its flank.
The brown horse makes its first overall appearance in 3:10 to Crazytown, when the contestants have to jump onto the horse from a hundred foot platform. The horse is standing on a painted target, drinking water out of a bucket, and hardly looks up from it. Most of the castmates have brutal landings, but do not hurt the horse. Gwen falls back-first onto the horse, which whinnies and looks up from the bucket when she lands. It remains stunned for a few seconds while Gwen falls off of it, but then goes right back to drinking its water. Trent attempts to land on the horse, but falls groin-first onto the fence. Harold lands on the horse, but it doesn't seem to notice. A few seconds later, Duncan lands on top of Harold, which the horse doesn't notice either. Owen almost lands on the horse, however, it looks up and quickly moves away at the last second, causing Owen to fall through the ground. Owen then chases the horse around and eventually mounts it. However, the horse is trembling and does not look like it can hold Owen. It continues to walk around a bit with Owen riding it. DJ, like Trent, attempts to land on the horse, but hits electrical wires instead. Lindsay and Beth land on the horse; however, since they had jumped at the same time, their combined weight makes the horse whinny loudly, fart, and then faint. After the horse recovers, Heather and Leshawna also attempt to land on the horse, but miss.
The horse also makes an appearance in The Princess Pride when Chris rides it into the main lodge. When Courtney is kidnapped by "the terrible toothless troll", the horse kicks Justin in the face with its hind leg. Chef Hatchet refers to the horse at one point as "Old Betsy".
One of the challenges in Mutiny on the Soundstage is a reverse challenge of the one in 3:10 to Crazytown, with the horse having to jump on the selected castmate. However, only Duncan had to do this as Beth passed it by answering a question about Trent's phobia correctly.
The horse makes its first Total Drama World Tour appearance in Super Happy Crazy Fun Time Japan. It is ridden by Harold, who is dressed like a samurai as part of Team Victory's commercial. Chef refers to it as a "sad donkey thing" when criticizing Team Victory's.
The horse appears once again in Awwwwww, Drumheller, where it is seen running out of the cargo hold of the Total Drama Jumbo Jet, alongside Ezekiel and many other animals of the Total Drama series, right before Sierra accidentally causes the plane to explode.
A horse appears next to Alejandro during I'm Gonna Make It when he falls off the train. Several different horses appear in a galloping herd in Planes, Trains, and Hot Air Mobiles as Heather and Alejandro race towards Mexico. Both contestants end up riding one of these horses in order to continue their non-stop race. These horses are shown to be much more agile and in better condition than the ones used in the previous challenges, possibly because they are not in ownership of the show.
Horses made a small appearance in I Love You, I Love You Knots when Chris rewards the winning team with take out order from Humpty Dumpty's Meatshack. Apparently, the restaurant serves horse meat which greatly disgusts the contestants (except Sugar). The contestants refuse to eat the dinner and eat the chicken instead.
In Hurl and Go Seek, Chris feeds the competitors "Juggy Chugs," horse meat combined with other ingredients and placed in a jug. The meat has been expired since 1976, and all who consume it are required to have their stomach pumped. The mascot of Juggy Chugs is a cartoon horse which gives a thumbs up at the end of the advertisement. Sugar claims that cartoon horses always do this, or they would be mistaken for the real thing.
In A Tisket, a Casket, I'm Gonna Blow a Gasket, the teams have to ride on horses from the castle to the gymnastic training center. The horses also appear in Last Tango in Buenos Aires, where one team member rides a horse to catch an emu.
- Total Drama: Revenge of the Island and Total Drama All-Stars are currently the only seasons to not feature a horse.